Web17 giu 2024 · Among the aptamer sequences identified, a hairpin-structured sequence SYL3 was optimized in length, resulting in aptamer sequence SYL3C. The Kd values of the SYL3C aptamer against breast cancer cell line MDA-MB-231 and gastric cancer cell line Kato III are 38 ± 9 and 67 ± 8 nM, resp., which are better than that of the full-length SYL3 … Web6 ago 2024 · The binding capabilities of Apta 2 and Apta 42 could not distinguish both cell types. At the same time, Apta 21 and Apta 99 showed a better binding capacity to ASC with dissociation constants (Kd) of 50.46 ± 2.28 nM and 72.71 ± 10.3 nM, respectively. However, Apta 21 showed a Kd of 86.78 ± 9.14 nM when incubated with FIB.
Selection and targeting of EpCAM protein by ssDNA aptamer
Web14 apr 2024 · The development of aptamers represents a therapeutic alternative with significant advantages; they are small RNA or DNA sequences, which by forming three-dimensional structures, can act as antibodies capable of binding to a given antigen with high recognition and affinity [15,16].Thus, the design of a functional aptamer to inhibit … Web21 lug 2014 · The first aptamer binding to human α-thrombin was described by Bock et al. [26] in 1992. This 15-mer DNA oligonucleotide (5′-GGT TGG TGT GGT TGG-3′) can … milc brighton
Selection of a Nuclease-Resistant RNA Aptamer Targeting CD19
Web9 mar 2024 · When incubated with the same concentration of the target analyte for the same period, the collected chronoamperograms showed comparable performances (Figure 2A, B), provided that the BNP binding aptamer and anti-BNP antibody used in the work have a dissociation constant (K d) of 9.45±0.23 nM and 0.37±0.02 nM, respectively, based on … Web13 gen 2024 · Therefore, we designed and fabricated bi-aptamer arrays (36 TBA15 and 36 HD22, Aptarray) on DNA origami with inter-aptamer distances of 5.4 nm (Supplementary Fig. 14) for efficient thrombin binding. WebThe Kd values of the four AMs were measured using cell‐ELONA and number 10 exhibited the highest binding force, with a Kd of 38.69 ± 10.51 nM (Figure 4D). The binding of fluorescein thiocyanate (FITC)‐labeled AM 10 (A10) to A549/DDP cells was dose‐dependently increased, as determined by flow cytometry (Figure 4E). new year resolutions for students work