Endofectin max转染试剂

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August undefined, 2024

WebItem EndoFectin™ Max Transfection Reagent; Company GeneCopoeia; Catalog Number EFM1004-02; This product is no longer available on Biocompare. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. About Biocompare

1分钟解锁两种转染试剂的差异 - 知乎 - 知乎专栏

Web核酸转染试剂. 转染试剂. Lipofectamine™ RNAiMAX 转染试剂. See the new Lipofectamine® RNAiMAX Transfection Reagent protocol —now optimized for efficiency, viability and … WebWhether it's raining, snowing, sleeting, or hailing, our live precipitation map can help you prepare and stay dry. rotor subotica

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WebPEI MAX 也叫线性化聚乙烯亚胺PEI 40000（Polyethylenimine Linear, MW 40000，是一款基于聚乙烯亚胺（Polyethylenimine L，PEI）的产品，是一种分子量为40000的瞬时转染试剂，带有高电荷阳离子，非常容易结合带负电荷的DNA分子，形成复合物，并转染到贴壁和悬浮细胞中，已广泛应用于科研和工业用途。 WebEndoFectinTM-MAX 转染试剂广泛适用于常见细胞系，如 HEK-293、 HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。. 该试剂即使在有血清存 … WebEndoFectin™ Expi293（为Expi293F悬浮细胞进行优化设计），货号：EF007，专注转染悬浮细胞293F,转染效率极高. § 特别为Expi293F悬浮细胞进行优化设计，配合Expi293F悬浮细胞使用，可提高实验效率； § 与 … rotors turned autozone

GeneCopoeia - EndoFectin™ Transfection Reagents 复能基因

Web制作DNA-EndoFectin™复合物： A.从冰箱取出DNA质粒、EndoFectin™Max转染试剂、无蛋白培养液（如Opti-MEMI™）恢复至室温。 B.使用之前将EndoFectin™Max转染试剂 … WebEndoFectin™ Max transfection reagent (1 mL) $295: Download: EF014: EndoFectin™ Max transfection reagent (3 mL) $589: EF001: EndoFectin™ Lenti transfection reagent (1 mL) $245: ... EndoFectin™ Expi293 transfection reagent (3 mL) $640 . Additional related clones. GLuc-ON™ Promoter Reporter Clones; rotors turned near me san ramon caWebsiRNA转染步骤—悬浮细胞. 6孔板实验为例：. 1、转染的当天，收集细胞离心，用含FBS的培养基重悬。. 2、在250μl的无血清生长培养基中加入100 pmole siRNA，柔和混匀。. 3、 Lipofectamine RNAiMAX使用前要先混匀，然后在250 μl生长培养基中加入5μl的Lipofectamine RNAiMAX进行 ... stranded deep wiki crafting

"WebTransfection-mediated cytotoxicity can mask the true phenotype of a target gene being studied, so minimizing the amount of reagent used in your transfections is a critical factor for successful RNAi experiments.. Lipofectamine RNAiMAX Transfection Reagent gives maximal knockdown and excellent cell viability across a 10-fold concentration range of … " - Endofectin max转染试剂

Endofectin max转染试剂

Lipofectamine™ MessengerMAX™ 转染试剂 - Thermo Fisher

WebDec 19, 2024 · Methods:we grew mouse primary renal tubular epithelial cells to approximately 50% confluence, transfected using EndoFectin™ Max (GeneCopoeia, China). H/R was used to mimic IRI in vitro. Briefly, cells were incubated in glucose-free medium in a tri-gas incubator (94% N2, 5% CO2 and 1.0% O2) at 37 °C for 6 hours. … Web广州天骏生物科技有限公司，代理品牌：美国GeneCopoeia, Affinity, Macklin上海麦克林，上海陶素TargetMol，北京四正柏，北京东方瑞利，美国爱马斯Ammex，Abcam，康宁Corning，Axygen，日本同仁CCK …

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WebLipofectamine™ RNAiMAX is a proprietary RNAi-specific cationic lipid formulation designed specifically for the delivery of siRNA and miRNA into all cell types. Lipofectamine™ RNAiMAX Transfection Reagent transfects a wide range of cell types (see figure). For gene silencing, Lipofectamine™ RNAiMAX Transfection Reagent's high-efficiency ... WebEndoFectin™Max转染试剂稀释至125μL，室温静置5分钟。） 5分钟后，轻柔混匀稀释的DNA与稀释的EndoFectin™Max转染试剂（注意：EndoFectin™Max试剂在进行稀释 …

WebDOTAP脂质体转染试剂 >99% (TLC), liquid, suitable for transfection; CAS Number: 144189-73-1; Synonyms: DOTAP硫酸甲酯,脂质体转染试剂，dotap,转染试剂，dotap,N-(2,3-Dioleoyloxy-1-丙基)三甲胺甲基硫酸甲酯,转染试剂; find Roche-11202475001 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich WebEndoFectin™ Max GeneCopoeia现推出的EndoFectin™ Max是基于脂质体转染原理的转染试剂，该试剂具有多种细胞的广泛通用性，经优化设计，在多种常用细胞系中表现优 …

WebEndoFectin Transfection Reagents Publications. 2024. Garcia-Morales, V., et al. (2024). The microRNA-7-mediated reduction in EPAC-1 contributes to vascular endothelial permeability and eNOS uncoupling in murine experimental retinopathy. Acta Diabetologica doi: 10.1007/s00592-017-0985-y [EndoFectin™] WebThe City of Fawn Creek is located in the State of Kansas. Find directions to Fawn Creek, browse local businesses, landmarks, get current traffic estimates, road conditions, and …

WebJul 7, 2016 · EndoFectin™ Max、Lipo2000分别以FLuc表达质粒转染几种低转染率细胞，通过检测荧光素酶的表达活性比较转染效率。图2. EndoFectin™ Max、Lipo2000分别对Hela细胞进行转染操作，通过计算转染后的细胞存活率比较细胞毒性。

WebNov 20, 2024 · For 1 μg of total plasmid DNA, dilute 3 μl of EndoFectin Max in 50 μl DMEM. The weight–volume ratio of DNA/EndFectin is 1:3. If the EGFP and mCherry constructs are cotransfected with integrin, the total amount of EndoFectin needs to be adjusted accordingly. 5. Mix the diluted DNA and EndoFectin and incubate at room … stranded deep tower flagWebEndoFectin™ MAX与Lipofectamine® 2000 (Invitrogen)对质粒和siRNA的转染效果. 分别借助两种转染试剂，将荧光素酶表达质粒及对应siRNA转染至两种常用细胞系（H1299 … rotor supplyWebCHO细胞的转染试剂。 Endofectin™-Max–最广谱的转染试剂，甚至适用于粘连细胞系和悬浮细胞系应用不同的转染试剂将表达EGFP的质粒转染导人胚肾293T 细胞，转染20小时 … rotors walmartWeb我们提供用于 DNA、siRNA、RNA 和蛋白递送的多种转染试剂，以及一系列最适合您转染实验的方案：. DNA 递送 ——瞬时和稳定转染用于研究基因功能和基因调节，基因产品的 … rotors turned wichita ksWebJun 22, 2011 · 使用目的. EndoFectin™ Max. ：一般的な細胞株（トランスフェクションが困難な細胞株を含む）に汎用的に使用できる脂質ベースの試薬. EndoFectin™ Expi293. ：一過性発現に使用される浮遊細胞株 Expi293F のトランスフェクションに最適. EndoFectin™ HepG2. ：導入困難な ... stranded deep xbox one controlsWebLipofectamine™ MessengerMAX™ 试剂有助于增加利用 GeneArt™ CRISPR 核酸酶 mRNA 通过高效转染成功切割和重组的可能性，最终实现基因修饰效率较大化以及简化下游过程。. 高效转染难转染细胞. Lipofectamine™ MessengerMAX™ 试剂设计用于转染更高量的 mRNA 到神经元以及广泛 ... rotors weightWebDilute EndoFectin Max reagent with the same protein-free diluents3. Use 2.0 to 4.0 µl of EndoFectin Max reagent per 1 . µg of DNA4. Add the diluted EndoFectin Max reagent to the DNA solution. Mix the solution gently by tapping the tubes. Incubate the mixture for 25 minutes at room temperature to allow the DNA-EndoFectin Max complex to form. 3. rotor surfacing