Hioecs
WebbFusobacterium nucleatum, an anaerobic oral opportunistic pathogen associated with periodontitis, has been considered to be associated with the development of oral … Webb13 maj 2010 · Cell culture and induction by benzo(a)pyrene[]The human immortalized oral epithelial cells (HIOECs), which were obtained from normal oral mucosa immortalized by transfection of HPV16 E6/E7 gene as previously described, were cultured in keratinocyte medium-SFM (Gibco, USA) and treated intermittently and gradually with 0.1–1.2 mg/ml …
Hioecs
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Webb16 juni 2015 · 人永生化口腔上皮细胞系（ human immortalizedoral epithelial cells,HIOECs） 是用人乳头瘤状病毒E6、E7 开放读码框架转染原代培养的正常口腔上皮细胞后建立的[7],HIOECs 具有与口腔黏膜上皮细胞相似的形态和生化特性，所建模型可被用于评价药物在口腔吸收机制的研究[8].人永生化皮肤角质形成细胞 HaCaT 是由 ... WebbHIOECs and SCC-9 cells were infected with Fusobacterium nucleatum at a MOI of 100 : 1, and (A) the mRNA and (B) protein expressions were assayed by qRT-PCR and western …
Webb19 nov. 2024 · When HIOECs were treated with [email protected] at the MBIC (50 μg/mL), they retained 60% cell viability, indicating that [email protected], even at high concentrations, were well tolerated. In contrast, the CHX group exhibited significant cytotoxicity compared to the [email protected] group, which was in line with the slow … WebbDownload scientific diagram (a) Representative images of fused cells between RFP-CAL-27 and GFP-HIOECs (100x and 200x). (b) Representative confocal images of fused …
WebbHIOECs were provided by Prof. Wantao Chen from Key Laboratory of Shanghai Oral Medicine, Shanghai Jiao Tong University, and grown in defined keratinocyte-SFM (Gibco) with growth supple-ment at 37°C and 5% CO2. F. nucleatum ATCC25586 was rou - tinely cultured on tryptic soy broth agar plates containing 5% Webb17 okt. 2024 · Our data showed that bleomycin can induce fibrosis in HIOECs and HGFs, as shown by the elevated levels of α-SMA (Figure S6A). However, after local injection of bleomycin into the DF of rats, our immunohistochemistry data showed no significant increase in α-SMA expression in the gingival tissues surrounding the DF (Figure S6B,C).
Webb21 feb. 2024 · HIOECs were kindly provided by Dr. Wantao Chen from Key Laboratory of Shanghai Oral Medicine, Ninth People's Hospital, Shanghai Jiao Tong University. HIOECs were cultured in Defined Keratinocyte-SFM (GibcoTM, Thermo Fisher Scientific Inc., MA, USA) with growth supplement in a humidified atmosphere (37°C, 5% CO 2).
Webb31 juli 2008 · Comparative proteomic analysis of all-trans-retinoic acid treatment reveals systematic posttranscriptional control mechanisms in acute promyelocytic leukemia. Harris MN , Ozpolat B , Abdi F , Gu S , Legler A , Mawuenyega KG , Tirado-Gomez M , Lopez-Berestein G , Chen X. Blood, (5):1314-1323 2004. MED: 15142884. how to switch iphone 11 onWebbThe HIOECs were cultured in defined keratinocyte serum-free medium (DK-SFM) (Gibco, CA, USA) in a humidified atmosphere of 5% CO 2 at 37°C. 2.3. CCK-8 Assay. HIOECs … reading voyage plus 3 답지Webb12 sep. 2024 · The cell cycle distribution of HIOECs was analyzed by flow cytometry. Additionally, the RNA levels of EGF, FGF-2, and TGF- β 1 of HIOECs were assessed … how to switch isa accountsWebb1 maj 2008 · The human immortalized oral epithelial cells (HIOECs), which had been obtained from normal oral mucosa immortalized by transfection of HPV16 E6/E7 gene … reading vq scansWebb5 apr. 2024 · HIOECs were kindly provided by Professor San-Gang He from the School of Stomatology, Wuhan University. The HIOECs were plated in 6-well plates and cultured in defined keratinocyte serum-free medium (Gibco, Carlsbad, CA, USA). When the HIOECs were grown to 80% confluence, ... reading vs listening comprehensionWebb19 nov. 2024 · When HIOECs were treated with Ag-MSNs@CHX at the MBIC (50 µg/mL), they retained 60% cell viability, indicating that Ag-MSNs@CHX, even at high concentrations, were well tolerated. In contrast, the CHX group exhibited significant cytotoxicity compared to the Ag-MSNs@CHX group, which was in line with the slow … reading vs audio booksWebbHIOECs. Target genes in cis and trans-acting of DElncRNAs were predicted for further functional analyses. The cis role of lncRNAs indicated their actions on neighbouring … how to switch iphones with family member