WebIt is often possible to detect macromolecules that form stable complexes with the target protein in a pull-down assay—when the pull-down is accomplished using immobilized … WebJun 15, 2015 · Although the current purification protocol negatively selects for rRNA (only ∼ 0.16% of reads are mapped to rRNA in the purified input poly(A) + RNA), the biotin …
超详细！手把手教你做RNA pull down - 知乎 - 知乎专栏
C. elegans strains (Supplementary Table 1) were cultivated using standard procedures. Worms were maintained at 24 °C on Escherichia coli OP50-seeded NGM plates59. The MosSCI injection strain EG6699 was kept at 16 °C for maintenance. The BS1080 gld-1 transgenic strain24 was a gift from Tim Schedl. … See more Probe design: Probes (3′-biotin-TEG modified 20 nt long DNA antisense oligonucleotides; metabion) tiling the gfp CDS or entire endogenous transcripts were designed essentially as described previously60. Probes … See more CLIP-qPCR was performed to validate candidate interactors identified with vIPR. For reproducible and efficient immunoprecipitation, we used transgenic C. elegans strains expressing GFP-tagged proteins, together with … See more For input and supernatant samples, 6–13 μg of protein were used for analysis by mass spectrometry. For pulldown elution samples, we roughly estimated the protein amount to be ~250 … See more Reversed-phase liquid chromatography (rpHPLC) was performed employing an EASY nLC II (Thermo Fisher Scientific) using self-made fritless C18 microcolumns (62; 75 … See more WebJul 25, 2024 · RNA replication is a key step in the infection cycle of RNA viruses. This process involves both virus- and host-encoded proteins, and takes place in endo … granite construction anchorage
pull down实验结果怎么看? - 知乎
WebFifty microliters of cell lysate without RNA pulldown were used as an input control. Fish assay. Prepare the probes. Then, a larger wet box was used to place the slices crosswise, and 10-µL probes were dropped on the sliced tissue. Add a coverslip, cover the wet box and incubate at 37°C for 12-16 h. WebIn the application, a long-chain non-coding RNA BC0009639 possibly closely related to tumor migration is screened and verified from a pair of lung cancer cell lines with different migration potency by using a genechip, proved by scratch experiment and Transwell experiment, the long-chain non-coding RNA BC0009639 can inhibit migration of tumor … WebRNA Pulldown是研究RNA与蛋白质相互作用的一种技术。. 利用生物素标记的RNA探针，通过与含有目的蛋白的溶液孵育，形成RNA-蛋白质复合物。. 复合物可以特异性结合磁珠，从 … granite construction aggregate plant